Fig. 1

Fig. 1

zepmutants acquire edema and display altered fluid clearance. (A) Compared to live WT siblings, zep mutant embryos have acquired an edematic phenotype that progresses in severity throughout the lifespan of the animal. In zep, the location of the pericardial cavity (black arrowhead) showed no edema at 48 hpf, while subtle edema was detected at 72 hpf which progressed in severity at 96 and 120 hpf. (B) Following microinjection of 40 kDa dextran-FITC into the vasculature at 72 hpf, WT siblings exhibited endocytosis by the proximal tubule (white box and inset) at 96 hpf, while zep embryos showed accumulation of fluorescence in edemic areas and the yolk (asterisk) and failed to exhibit proximal tubule uptake. Embryos are shown in lateral views.