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Fig. S1
Further assessment of cell proliferation and death inzepmutants. (A) The number of pH3+ cells was quantified in the cervical region of WT and zep embryos at the 24 hpf stage. The quantity of pH3+ cells was not statistically different as assessed by student T-test. (B) Whole mount AO staining and (C) anti-Caspase-3 immunostaining of the tail region in WT and zep embryos revealed similar levels of cell death, which was assessed by student T-tests (D, E) respectively.
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Reprinted from Developmental Biology, 428(1), Kroeger, P.T., Drummond, B.E., Miceli, R., McKernan, M., Gerlach, G.F., Marra, A.N., Fox, A., McCampbell, K.K., Leshchiner, I., Rodriguez-Mari, A., BreMiller, R., Thummel, R., Davidson, A.J., Postlethwait, J., Goessling, W., Wingert, R.A., The zebrafish kidney mutant zeppelin reveals that brca2/fancd1 is essential for pronephros development, 148-163, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.