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Fig. S1
Biliary epithelial cell remodeling during intrahepatic biliary network formation in zebrafish. (A-D) Projected confocal image of a Tg(Tp1- MmHbb:EGFP)um14 liver visualized for GFP (green) and DAPI (pseudo-color blue) at 3 (A), 4 (B), 5 (C) and 6 (D) dpf. GFP marks the intrahepatic biliary network. Biliary epithelial cells form clusters (white arrowheads) within the intrahepatic biliary network in wild-type larvae at 3 and 4 dpf. However, these cells separate after 5 dpf. (E) The percentage of biliary epithelial cells that are not clustered with other biliary epithelial cells.
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