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Fig. 7
Change of the expression levels of miR-206, Rtn4al, Cxcr4a and Thbs3a resulted in the failure of p-FAK to concentrate at the intersomitic boundary of defective boundary cells. (a–c) In the WT embryos at 20 hpf, p-FAK signal was concentrated at the position of the intersomitic boundary; (d–f) knockdown of miR-206, (g–i) overexpression of rtn4al, (j–l) knockdown of cxcr4a and (m–o) overexpression of thbs3a. DAPI labelled with blue fluorescent signal was used to mark the nucleus, while green fluorescent signal was used to label p-FAK. (c,f,i,l,o) Two fluorescent signals were merged. White arrowheads indicate that p-FAK did not concentrate at the intersomitic boundary of defective boundary cells.