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Fig. S3
Related to Fig 1. Chemical ablation of macrophages using the NTR/Mtz system.
(A) Schematic representation and treatment schedule of the chemical ablation strategy based on the nitroreductase (NTR)/ metronidazole (Mtz) system, shown for macrophage-specific ablation. See Materials and methods for details. (B) Representative fluorescence images of non-treated and Mtz-treated mpeg1/umn+ embryos, showing the efficacy of the Mtz treatment. Residual red fluorescence in treated embryos represented apoptotic cells. Scale bar, 0.5 mm. (C) Quantification of macrophage numbers in mpeg1/umn+ embryos (untreated or treated at 34 hpf with 5mM Mtz) at 0, 15 and 39 hours after treatment as indicated in (A). The efficacy of macrophage ablation by Mtz treatment was evaluated by pixel counting. The average macrophage numbers of two independent experiments (n = 6) are shown. ns, non-significant, ** p ≤ 0.01, **** p ≤ 0.0001. See material and methods for statistical analysis used.