ZFIN Image: Murphy et al., 2017, Fig. 12

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Figure Caption

Fig. 12

Suppression of cell death has little effect on disrupted eng2a expression in brd2aMO morphants.

TUNEL assay and in situ hybridization to eng2a RNA in brd2aMO morphants in which cell death is suppressed by A–L) treatment with pan-caspase inhibitor Q-VD-OPh; or M–X) treatment with p53MO co-injection. For pan-caspase study: wildtype DMSO (A,B,C), Q-VD-OPh (D,E,F), brd2aMO (G,H,I), and brd2aMO + Q-VD-OPh (J,K,L) treated embryos were assessed for apoptotic nuclei by TUNEL assay followed by laser scanning confocal microscopy (brightfield A,D,G,J; darkfield B,E,H,K); and for eng2a expression by in situ hybridization (C,F,I,L). brd2aMO morphants exhibit reduced/absent eng2a expression in the anterior hindbrain domain compared to controls, regardless of the suppression of cell death by pan-caspase inhibition (arrows in I and L versus C and F). Refer to E for quantitative analysis.

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Acknowledgments

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Reprinted from Mechanisms of Development, 146, Murphy, T., Melville, H., Fradkin, E., Bistany, G., Branigan, G., Olsen, K., Comstock, C.R., Hanby, H., Garbade, E., DiBenedetto, A.J., Knockdown of epigenetic transcriptional co-regulator Brd2a disrupts apoptosis and proper formation of hindbrain and midbrain-hindbrain boundary (MHB) region in zebrafish, 10-30, Copyright (2017) with permission from Elsevier. Full text @ Mech. Dev.