ZFIN Image: Reinardy et al., 2015, Fig. 2

Image

Figure Caption

Fig. 2

Tie1 and Thr794 are required for proper embryonic vascular development in zebrafish.

a-e Zebrafish embryos expressing Fli1:EGFP were injected with a control or Tie1 morpholino (MO) with or without mRNA encoding WT or T794A (TA) mutant Tie1 and effects on vascular development were assessed. b Zebrafish injected with a control MO develop normally and resemble uninjected (Control) embryos. c Tie1 MO-injected embryos display vascular abnormalities. d Co-injection of Tie1 MO and WT mouse Tie1 mRNA rescued the MO phenotype. e Injection of T794A mutant mouse Tie1 mRNA alone resulted in vascular abnormalities similar to those of MO-injected embryos. Arrows indicate blunted intersomitic vessel formation. f Quantification of mutant embryos after injection of Tie1 MO with WT or T794A mutant mRNA, P = 3.0x10^-19 by Chi squared analysis. g Quantification of mutant embryos after injection of Tie1-WT or -TA mRNA alone, P = 1.3x10^-18 by Chi squared analysis.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One