ZFIN Image: Kawahara et al., 2016, Fig. 2

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Figure Caption

Fig. 2

Altered skeletal muscle in sil1-morpholino injected fish.

A: Left panels are pictures under bright field, and left panels are birefringence assay (BR). Abnormal structure of muscle is obvious in sil1 morpholino injected fish (MO1 and MO2) compared to control (CMO) under birefringence assay. B: Histogram of the percentage of normal, affected, and dead fish by morpholino-injection. Injection of 3 ng of MO1 or MO2 resulted in approximately 39.0% and 21.8% of injected embryos exhibiting reduced birefringence, respectively. C: Restoration of sil1 morphant with co-injection of fish sil1 mRNA (50 pg). Histogram of the percentage of dead, affected fish of morphants and recovered fish. Co-injection of zebrafish sil1 mRNA with each MO (3 ng) rescued the phenotypes. White bar shows normal %, gray shows affected % and black shows dead fish %. Non: non injected fish, MO1: MO1 injected fish, MO2: MO2 injected fish.

Figure Data

Phenotype details

Acknowledgments

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