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Fig. 5
Expression of DNγ2 in MG of Undamaged Retina Causes Increased Proliferation
(A) Model illustrating effects of electroporation of DNγ2 into MG on proliferation.
(B–E) A GFAP:mCh-DNγ2 construct was electroporated into one retina of undamaged Tg(gfap:GFP)mi2001 fish. GFP expression (B), mCherry expression (C), and staining for PCNA (D) all co-labeled in the same cell (E). Scale bar, 100 μm.
(F) Total number of PCNA-expressing cells was measured for pA electroporation (n = 9 eyes analyzed) and DNγ2 (n = 12). Two-tailed Student’s t test was used; error bars denote SD; ∗p < 0.05.
See also Figure S7.
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