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Fig. 2
In the absence of VE-cad, several filopodial contacts are required to initiate cell-cell contact formation. (A-F) Still images from Movie 2 of a VE-cad morphant Tg(fli1ep:gal4ff)ubs3, Tg(UAS:EGFP-UCHD)ubs18, Tg(kdrl:mCherry-CAAX)s916 embryo at around 32 hpf, with anterior to the left. Single channels are shown in inversed contrast (green is EGFP-UCHD and red is mCherry-CAAX on left and right, respectively) and the merge is shown in the middle. Red arrowheads point to filopodial interactions or cell bodies touching in (E); green arrowheads point to cell-cell bridges and forming junctions in (E) and (F). Scale bar: 10 µm.