Fig. 5

Fig. 5

Akt2 mutants display impaired glucose homeostasis. (A) Pancreas development in homozygous (MZakt2^−/−) larvae was indistinguishable from their wildtype siblings, as revealed by insulin (endocrine pancreas) and trypsin (exocrine pancreas) staining; try, trypsin. (B) Blood glucose concentration of MZakt2^−/− mutants (3-months old; n = 5) was higher than that of their wildtype siblings (3-months old; n = 5) after being fully fed. Zebrafish were fully fed for 15 min, and blood glucose concentration was measured after 8 h. (C) Blood glucose concentration of MZakt2^−/− mutants (3-months old; n = 3) was higher than that of their wildtype siblings (3-months old; n = 3) after intraperitoneal injection with glucose solution. Zebrafish were intraperitoneally injected with glucose solution, and blood glucose concentration was measured after 4 h. (D) The mRNA level of insulin in MZakt2^−/− mutants (3-months old; n = 6) was much lower than that of their wildtype siblings after being fully fed. Zebrafish were fully fed for 15 min, and total RNA was extracted from heart, muscle, and liver (ventral lobe) after 4 h. (E) The mRNA level of insulin in MZakt2^−/− mutants (3-months old; n = 6) was much lower than that of their wildtype siblings after fasting for 2 d. (F) The protein level of insulin in the liver-pancreas of MZakt2^−/− mutants (3-months old; n = 6) was lower than that of their wildtype siblings after being fully fed. Zebrafish were fully fed for 15 min, and protein was extracted from the liver-pancreas after 4 h and detected via the ELISA method. (G) The mRNA level of glut1 in MZakt2^−/− and Makt2^+/− embryos (5 dpf, ♀akt2^−/− × ♂akt2^+/−) was lower than that of wildtype embryos (5 dpf, akt2^+/+ × akt2^+/+). (H) Immunofluorescent staining for insulin expression in pancreas of akt2^+/+ and akt2^−/−; the magnification of images is the same.