|
Fig. 1
Caf mutants display a coiling phenotype as early as 23 hpf.
A) One full coil completed by a 24 hpf wild type (lama2+/+ and lama2+/-; WT) zebrafish embryo. B) Number of coils of WT siblings and caf (lama2-/-) mutants over 30 seconds when left in their chorions. There is no significant difference in the coiling abilities of caf mutants and their WT siblings at 22 hpf (WT sibs: 1.071 ± 0.3391, n = 14; cafs: 1.000 ± 0.7071, n = 4; p>0.9999), 23 hpf (WT sibs: 0.9286 ±0.3987, n = 14; cafs: 1.000 ± 1.000, n = 2; p>0.9999) or 24 hpf (WT sibs: 1.133 ± 0.2906, n = 15; cafs: 1.222 ± 0.3643, n = 9; p>0.9999). C) Number of coils of WT siblings and caf mutants in the 15 seconds immediately after manual dechorionation. At 22 hpf, there was no significant difference in number of coils completed by WT siblings and caf mutants (WT sibs: 3.654 ± 0.7685, n = 26; cafs: 3.250 ± 1.031, n = 8; p>0.9999). However, cafs completed significantly less coils than their WT sibs at 23 hpf (WT sibs: 7.444 ± 0.5587, n = 72; cafs: 3.476 ± 0.5921, n = 21; p = 0.0194) and 24 hpf (WT sibs: 10.71 ± 0.4436, n = 163; cafs: 2.936 ± 0.6646, n = 47; p<0.0001). Bars represent mean ± SEM.