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Fig. S1
H3.3-bio is Present Sub-stoichiometrically to Endogenous H3.3 Protein (related to Figure 1)
(A) cmlc2:H3.3-bio does not drive expression (green) in epicardium and endocardium. Both non-muscle cell types are recognized by an antibody against Raldh2 (arrows; red). Scale bar, 50 μm.
(B) Endogenous H3.3 is expressed in excess of transgenic H3.3-bio, which is undetectable using anti-H3.3 (lane 1). However, H3.3-bio can be detected from whole hearts using anti-FLAG (lane 2). Immunoprecipitation of H3.3-bio chromatin using the FLAG antibody enriches both forms of H3.3 (lane 3).
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Reprinted from Developmental Cell, 40, Goldman, J.A., Kuzu, G., Lee, N., Karasik, J., Gemberling, M., Foglia, M.J., Karra, R., Dickson, A.L., Sun, F., Tolstorukov, M.Y., Poss, K.D., Resolving Heart Regeneration by Replacement Histone Profiling, 392-404.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell