ZFIN Image: Zhu et al., 2017, Fig. S1

Image

Figure Caption

Fig. S1

vegfab is not involved in early cardiovascular development in zebrafish. (A) Open boxes and black lines denote exons and introns of the early coding and non-coding region of vegfab. MO in red mark the target sites of the vegfab translation blocking. P1 and P2 indicate the sites of primers; (B) Fragments of vegfab sequences were amplified using the indicated primers. In vegfab morphant embryos, the normal vegfab RT-PCR product was not detected (lane 3). β-actin cDNA was also amplified from each sample as an internal control; (C,D) The expression of vegfab during early embryogenesis cannot be detected. Scale bar: 200 μm (C); 500 μm (D); (E) The heart development is almost normal with 10ng doses of vegfab MO at 96 hpf under the background of Tg(myl7:mCherry)/Tg(kdrl:EGFP) transgenic fish. Scale bar: 50 μm.

Acknowledgments

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