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Fig. 6

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ZDB-IMAGE-170413-58

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Figures for Carroll et al., 2014

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Figure Caption

Fig. 6

Blocking Esr Signaling Increases VEGF Activity and Alters Trunk Vasculature Patterning

(A) VEGFAa expression was enhanced after inhibition of endogenous estrogen activity via the pan-Esr antagonist ZK164015 (18/32).

(B) gfp expression in VEGF:GFP reporter embryos was reduced by E2 and enhanced by ZK (n > 25).

(D) dusp5 levels were reduced by E2 treatment but elevated after Esr antagonism (n > 20).

(E) Arterial ephrinB2 expression in the fli1+ vasculature was enhanced after ZK-mediated inhibition of estrogen signaling (26/38).

(F) tbx20 expression in the roof of the aorta was substantially increased by ZK (20/36).

(G) Venous flt4 was expanded dorsally toward the col2a+ hypocord by E2 but reduced in expression by ZK exposure (14/36).

(H) The venous marker dab2 was similarly regulated as determined by WISH (n > 20).

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Reprinted from Developmental Cell, 29, Carroll, K.J., Esain, V., Garnaas, M.K., Cortes, M., Dovey, M.C., Nissim, S., Frechette, G.M., Liu, S.Y., Kwan, W., Cutting, C.C., Harris, J.M., Gorelick, D.A., Halpern, M.E., Lawson, N.D., Goessling, W., North, T.E., Estrogen defines the dorsal-ventral limit of VEGF regulation to specify the location of the hemogenic endothelial niche, 437-53, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell