Fig. 7
Manipulation of endogenous Wnt signaling affects the size of the nkx2.5+ and tcf21+ domains. (A, B, D, E) Tg(nkx2.5:ZsYellow);Tg(tcf21:nucEGFP) control, Tcf7l1 depleted, and Wnt8a depleted embryos at the 18 s stage. Tcf7l1 depletion expands the total anterior tcf21+ progenitor domain length (green brackets) as well as the overlapping expression (yellow brackets) within the ALPM. (C) Graph depicting measurements of the total tcf21+ progenitors and overlap with nkx2.5+ progenitors from Tcf7l1 depleted embryos. Control embryos n = 10, Tcf7l1 depleted embryos n = 9. (D, E) Wnt8a depletion reduces the overall length of the tcf21+ domain and the overlap with the nkx2.5+ progenitor domain. (F) Graph depicting measurements of the total tcf21+ progenitors and overlap with nkx2.5+ progenitors from Wnt8a depleted embryos. Control embryos n = 8, Wnt8a depleted embryos n = 10. Green brackets - anterior tcf21+ progenitors. Red brackets - nkx2.5+ progenitors. Yellow brackets – regions of overlap between tcf21+ and nkx2.5+ domains. Scale bar indicates 100 μm.
Reprinted from Mechanisms of Development, 143, Mandal, A., Holowiecki, A., Song, Y.C., Waxman, J.S., Wnt signaling balances specification of the cardiac and pharyngeal muscle fields, 32-41, Copyright (2017) with permission from Elsevier. Full text @ Mech. Dev.