IMAGE

Fig. S3

ID

ZDB-IMAGE-170315-11

Genes

EGFP
gpr183a

Source

Figures for Zhang et al., 2015

Image

Figure Caption

Fig. S3

gpr183 is expressed in the hemogenic endothelium (HE) after 24 hpf, and is essential for adult hematopoiesis in zebrafish.(A) Time-course expression of gpr183 ranging from one-cell stage to 36 hpf shows that gpr183 is not expressed before 24 hpf. (B) Frozen sections of the embryos after WISH show that gpr183 is expressed in the AGM at about 26 hpf. (C) gpr183 expression in sorted ECs, HEs and HSPCs at 28 hpf by qPCR. Error bar, ±s. d. *p<0.05, ***p<0.001. (D) gpr183 expression using double fluorescence in situ hybridization (dFISH) in 30hpf Tg(fli1a:eGFP) embryo. (E) gpr183 is not expressed in gpr183^-/- embryos.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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