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Fig. 2 S2
Expression of oxt and its receptor by PT OXT neurons.
(A) Whole-mount in situ hybridization with DIG-labeled RNA probe directed against the oxt followed by a long (e.g. to saturation) development to NBT-BCIP colorimetric substrate. The specimen was then subjected to immunostaining with an anti-tyrosine hydroxylase (TH) antibody to detect dopaminergic neurons that serves as a landmark for the position of the posterior tuberculum (PT). Arrowheads in the left and right (zoomed in) panels indicates the position of PT OXT neurons expressing low but detectable levels of oxt mRNA. Scale bar, 100 µm. (B) A representative image (single confocal plane) of a transgenic Tg(oxt:egfp) larvae, which was subjected to fluorescent in situ hybridization with an oxytocin receptor (oxtr) mRNA probe (red) followed by immunostaining for EGFP (green). PT OXT cells that express oxtr are indicated by arrowheads. Scale bar, 20 µm.