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Fig. S6
zeb1a and foxo1a are expressed in the LPM during development, although not exclusively. Embryos were probed for endogenous zeb1a a) and foxo1a b) expression by WISH. At 24hpf zeb1a and foxo1a probes show positive staining in the gut region throughout the embryo. Transverse vibratome sections of posterior trunk region at 48hpf showed zeb1a and foxo1a mRNA localized in the area of the gut where LPM are supposed to differentiate in iSMCs (arrows).
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