ZFIN Image: Hamm et al., 2016, Fig. 3

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Figure Caption

Fig. 3 Mesenchymal Sema3d acts as a repulsive cue for CCV outgrowth. (A–C) Global sema3d overexpression reduces CCV width. (A) sema3d mRNA–injected Tg(kdrl:EGFP)s843 embryos exhibit reduced CCV width at 30 hpf. (B) sema3d expression was induced by consecutive heat shocks (hs) at 16 and 19 hpf (each for 1 h at 39°C) in Tg(hsp70:sema3dGFP), Tg(kdrl:HRAS-mCherry)s896 embryos. sema3d-overexpressing embryos show a thinner CCV at 30 hpf. (C) Quantification of CCV front width (each n = 18). Error bars indicate SD. (D) Mosaic sema3d k.d. through sema3d MO injection into one of 16 cells in Tg(kdrl:EGFP)s843 embryos (magenta, coinjection with DiI for cell tracking). Partial loss of sema3d in the mesenchyme (white asterisks) induced ECs (white arrowhead) to migrate into the mesenchyme (n = 3), indicating that sema3d acts as a repulsive cue in the mesenchyme next to the CCV. Ctr MO-injected mesenchymal cells (white asterisks in right panel) did not induce ectopic EC migration. ***, P < 0.001.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Biol.