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Fig. 10 Collective migration and cell morphology of the LLP are impaired in sema3d morphants. (A) Sema3d antibody staining and in situ hybridization (ISH) for sema3d showed Sema3d expression in the LLP and its deposited neuromasts (white arrowheads). (B) LLP migration distance is reduced in sema3d morphants at 24 and 30 hpf in Tg(-8.0cldnb:lynEGFP)zf106 embryos (LLP indicated by white arrowheads). (C) Deposition of neuromasts (pink arrowhead) is impaired in sema3d morphants at 30 hpf. (D) Organized LLP rosette structure (pink arrowheads) and cell morphology are impaired in sema3d morphants at 24 and 30 hpf.