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Fig. S4
Cross-regulation of prrx1a/b and hand2 expression.
In hand2 mutants, expression of prrx1a (A, B; magenta) is slightly upregulated (arrowhead) in ventral cells, and prrx1b (C, D; magenta) is unaltered. (E, F) hand2 expression (magenta) is unaffected in prrx1a; prrx1b mutants. dlx2a (green) marks all arch NCCs. (G-J) hand2 expression (magenta) is not rescued in prrx1a; prrx1b; edn1 triple mutants (J) compared with edn1 single mutants (I). Dashed lines indicate approximate arch boundaries. Scale bar = 20 μm.
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