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Fig. S1
Mounting and imaging strategy of mural cell transgenic lines. (A) Embryos were mounted in 0.9% low melt point (LMP) agarose at the desired time point of imaging. Confocal stacks were acquired through the zebrafish at a 1.5 μM step size. Typically due to limitations of penetrance of lasers with confocal microscopy, one side of the embryo (the side closest to the light source) is imaged in greater detail. Excess slices from imaging are trimmed to only include the slices that include the dorsal aorta. It is key for trimming for the embryo to have been mounted as flat as possible.
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