ZFIN Image: Stratman et al., 2017, Fig. S9

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Figure Caption

Fig. S9

Co-staining of vascular and basement membrane proteins shows vascular enrichment. (A-C) Collagen IV with vascular co-immunostaining: column 1- transmitted light image of the region of interest; column 2- collagen IV immunolabeling (pseudocolored red); column 3- immunolabeling of GFP expressed by the Tg(fli1a:GFP) zebrafish line (pseudocolored green); column 4- merge of the collagen IV and vascular images. Three conditions were analyzed: No Heat Shock Controls (A), Heat Shock Controls (B), and pdgfrDN- Heat Shock (C). (D-F) Fibronectin with vascular co-immunostaining: column 1- transmitted light image of the region of interest; column 2- fibronectin immunolabeling (pseudocolored green); column 3- immunolabeling of GFP expressed by the Tg(fli1a:GFP) zebrafish line (pseudocolored red); column 4- merge of the collagen IV and vascular images. Three conditions were analyzed: No Heat Shock Controls (D), Heat Shock Controls (E), and pdgfrDN- Heat Shock (F). Representative images were chosed from 3 independent rounds of immunostaining.

Acknowledgments

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