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Fig. 2
Loss of Zar1 causes all-male phenotype in zebrafish. (A) Analysis of genders of zar1 homozygotes (homo), heterozygotes (hetero) and wild-type siblings. (B-D) Histological analysis of gonads of zar1 homozygotes and sibling controls by H&E staining. About half of the gonads (20/37) from wild-type and zar1 heterozygotes were ovaries (B) while the other half (17/37) were testes (C). All the 13 gonads from zar1 homozygotes were testes (D). (E) Schematic diagram of the Tg(zp3b:zar1) transgenic construct. egfp coding sequences were placed under control of the cmlc2 promoter to help visually identify transgenic fish. (F) Gender analysis of zar1−/− homozygotes with or without EGFP signal, indicating Tg(zp3b:zar1) transgene. Females were recovered only from zar1−/− homozygotes with the Tg(zp3b:zar1) transgene. (G,H) H&E staining of ovaries of zar1+/− heterozygotes and zar1−/− homozygotes on the Tg(zp3b:zar1) transgenic backgrounds. Ovaries from zar1−/− homozygotes rescued with the Tg(zp3b:zar1) transgene are normal histologically. Tg(zp3b:zar1), Tg(zp3b:zar1,cmlc2:EGFP); sg, spermatogonia; sc, spermatocytes; sp, sperm; I,II,III, oocyte stage I, II or III. Scale bars: 40 μm.