ZFIN Image: Rydeen et al., 2016, Fig. S9

Image

Figure Caption

Fig. S9

Increased mmp9 expression in Cyp26-deficient embryos is not due to an increase in macrophage and neutrophil numbers.

(A,B) ISH of mmp9 in control and Cyp26-deficient embryos at 48 hpf. (C) RT-qPCR of mmp9 expression in control, Cyp26c1-depleted, gir mutant, and gir+c1 embryos. (D,E) ISH of mfap4 in control and Cyp26-deficient embryos at 48 hpf. (F) Graph depicting quantification of average macrophage number over the yolk sac (n = 15 per group). (G,H) ISH of mpx in control and Cyp26-deficient at 48 hpf. (I) Graph depicting the average number of neutrophils over the yolk sac (n = 10 per group). Error bars are SEM. Lateral view, anterior left (A,B), frontal view, anterior up (D,E,G,H); n>20 embryos per group (A,B,D,E,G,H). Scale bars: 50 mm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.