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Fig. S4
tbx2a/b morpholino-knock down did not inhibit mesoderm formation. Embryos injected with tbx2ab MO at the 1-cell stage and fixed at the 3 ss. Whole mount in situ hybridization was then performed with a multitude of mesodermal markers. When compared to wild-type controls, the domains of these markers in morphants were not noticeably different, therefore suggesting that tbx2a/b is specifically active in pronephros segment cell fate choiceg.
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Reprinted from Developmental Biology, 421(1), Drummond, B.E., Li, Y., Marra, A.N., Cheng, C.N., Wingert, R.A., The tbx2a/b transcription factors direct pronephros segmentation and corpuscle of Stannius formation in zebrafish, 52-66, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.