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Fig. 5
Generation of a ttn.2/1 near-null mutant by targeting two highly used exons. (A) Schematic representation of the ttnxu071 mutant. Arrows indicate locations of mutations. (B) Analysis of protein extracts from 2 dpf WT larvae and ttn.2xu065, ttn.1xu066 and ttnxu071 mutants. (C) qPCR with primers targeting ttn.2 and ttn.1, respectively. Means±s.d., N=5. *P<0.05, ** P<0.01. (D) The Z-disk structure marked by α-Actinin was disrupted in the ttnxu071 mutant. (E) Electron micrographs of somites of 2 dpf WT and ttnxu071 mutant larvae. (F) Z-disk structure was normal in ttn.2xu065/+ and ttn.1xu066 at 2 dpf, but sarcomere disarray was noted in ttnxu071/+ and ttn.1xu066. Scale bars: 20 μm (D,F) and 1 µm (E).