ZFIN Image: Takeuchi et al., 2015, Fig. 4

Image

Figure Caption

Fig. 4

Slit may not be involved in the axogenesis of GCs.

Slit2 was overexpressed using the hsp70l:Slit2-GFP; pou4f3:Gal4 UAS:GAP43-GFP (B-G), or hsp70l:Slit2-GFP (H-K) lines during development of the RGC and GC axons (3/4/5 dpf). The structure of the RGC and GC axons was examined at 5 dpf. Slit2-GFP was detected after 40 min of heat shock (A, lower panel). (B-G) Heat shock-induced overexpression of Slit2-GFP at 3 dpf induced abnormal axonal projections (trespass) of the RGCs (n = 2/2, indicated by an arrowhead in G). The RGC axons were visualized by immunohistochemistry with an anti-GFP antibody. Control non-induced (B, C, D) and heat-shocked larvae (E, F, G). Low- (B, E) and high-magnification dorsal views (C, F), and lateral views (D, G) of the tectum. (H-K) Heat shock-mediated overexpression of Slit2-GFP at 3 dpf did not affect the formation of GC axons (n = 10/10, J, K). The larvae were stained with anti-Vglut1 (GC axons, magenta) and parvalbumin7 (Pvalb7, PCs, green) antibodies. Overexpression at 4 or 5 dpf also did not affect the GC axons (S4 Fig). Statistic analysis is shown in S3 Table. Scale bars: 1 mm in A; 50 μm in E (applied to B), 40 μm in F (applied to C); 40 μm in G (applied to D); 100 μm in J (applied to H); 40 μm in K (applied to I).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.