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Fig. S13
Inhibition of FAK does not affect formation of GC axons.
Effect of an FAK inhibitor PF–573228 on axogenesis of GC axons. Wild-type larvae were untreated (A, B, 1% DMSO) or treated with 1 μM (C, D), 3 μM (E-H), 10 μM (I-L) and 30 μM (M-O) PF–5773228 (in 1% DMSO) from 10 hpf to 5 dpf. The resultant 5-dpf larvae were stained with anti-Vglut1 antibody. IC50 (half maximal inhibitory concentration) of PF–573228 is 30–100 nM [46] and 10 μM of PF–573228 was reported to sufficiently inhibit FAK in zebrafish embryos [45]. Scale bars: 100 μm in A (applied to B-O).
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