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Fig. 2
kcng4b is expressed in embryos but not in adults. (A,B) RT-PCR detection of kcng4b in embryos (A,A′) but not in adults (B). (C) Antisense WISH detected expression of kcng4b in the ventricular zone (24 hpf). (C′) Zoomed image, lateral view. (C″) Zoomed image of spinal cord, dorsal view. (D) kcng4b sense probe detected no signal. (E,F) Two transverse cryosections (15 µm, oblique, midbrain). (G,H) 30-48 hpf, kcng4b transcripts in the ventricular system, otic vesicle (G″,H′), anterior and posterior eye chambers, lenses (H′), and in intersegmental vessels (H″). In G,H, eyes were removed; G′,G″ is a flat mount with yolk removed. Inset in H′ shows a zoomed image of the lens. (I) kcng4a transcripts in sensory neurons and lens at 30 hpf. (I′) Zoomed image of the head (box) with lens (arrowhead) and trigeminal ganglion (arrow). (I″) Zoomed image of the spinal cord (box), sensory Rohon-Beard neurons (RB; arrows). cc, central canal; F, forebrain; H, hindbrain; isv, intersegmental vessels; M, midbrain; ov, otic vesicle.