ZFIN Image: Shen et al., 2016, Fig. 1

Image

Figure Caption

Fig. 1

Insertion of the GBT cassette into the second intron of zebrafish kcng4b causes a mutation. (A-D) Homozygous mutants show a clump of cells in the third ventricle (30 hpf, B,D,D′, white arrow) and hydrocephalus (48 hpf, F,G, black arrow and arrowheads). (H) TAIL-PCR mapped the insertion at position 57,917,547 on Chr. 7 in the (+) orientation (Zv9). (I) Insertion into the kcng4b locus truncates the full-length transcript. kcng4b^−/− expresses the truncated transcript-kcng4b* consisting of one coding exon. (J) RT-PCR analysis demonstrates a reduction of kcng4b transcripts in kcng4b^+/− and their absence in kcng4b^−/− (30 hpf). Actin was detected as a loading control. (A,B) Frontal views, (C-G) lateral views, anterior to the left. E, exon; wt, wild type; A and B, primers as detailed in the Materials and Methods.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development