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Fig. S1
Detection of endogenous Wdr68 and ectopic GFP-Wdr68 fusion protein in zebrafish embryos.
A-D) Immunofluorescence detection of endogenous Wdr68. A) Wdr68 expression in a wildtype 15-somites stage embryo raised at 32°C. B) a wdr68 mutant lacking Wdr68 protein. C) Wdr68 expression in a wildtype prim-5 stage embryo raised at 32°C. D) a wdr68 mutant lacking Wdr68 protein. E-F) live imaging of GFP fluorescence in Tg(hsp70l:GFP-wdr68)csu9 animals. E) transgenic animal with the only detected GFP expression coming from the cmlc:eGFP marker for transgenesis. F) same animal as in E but after a 0.5 hour heat shock (HS) at 39°C followed by 7 hours of recovery at 28.5°C. G) western blot analysis of Tg(hsp70l:GFP-wdr68)csu9 animals after various lengths of heat shock exposure. Panel G1) GFP-wdr68 expression is induced by heat shock. Panel G2) β-tubulin expression was used as a loading control and did not differ substantially between lanes.