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Fig. S1
Apoptosis induction by EtOH and 4OHT treatment in zebrafish larvae. (A) Treatments (0.5% EtOH, 5μM 4OHT, 3μM Staurosporine, or non-treatment) were started using wild type zebrafish embryos at 24hpf followed by apoptotic cell detection with acridine orange staining 10hrs later. Whereas Staurosporine treatment induced significant numbers of apoptotic cells, both EtOH- and 4OHT-treated embryos showed similar signals to those obtained in non-treated specimens. (B) Each bar in the graph indicates the relative number of acridine orange positive dying cells throughout the spinal cord region in relation to apoptotic cells in untreated embryos. Counting of the number of apoptotic cells was performed using confocal z-stack images of the spinal cord from 5 independent embryos in each group. * <0.01: p value is lower than 0.01 by Student´s t-test.