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Fig. 4
Caspase8ERT2-mediated apoptosis elicits no acute adverse effects on granule and eurydendroid cells. (A) Schematic of zebrafish cerebellar neurons and circuits. (B-D) Heteorzygous PC-ATTACTM fish were crossed with carriers of the Tg(gata1:egfp) strain marking granule cells by green fluorescence (B,C) or with Tg(olig2:egfp) carriers displaying green fluorescent eurydendroid cells (D). Double transgenic 4 dpf larvae were treated with EtOH (B) or 4OHT (C,D) for 16 h to induce PC ablation followed by confocal microscopy image recording. (C) gata1:egfp-expressing GCs, somata (arrows) and their axonal projections – the parallel fibers (asterisks) – showed no signs of cell death, in contrast to fragmented PCs after 4OHT treatment. (D) The same was true for olig2:egfp-expressing eurydendroid cells. Scale bars: 20 µm. BC, basket cell; CF, climbing fiber; EC, eurydendroid cell; GC, granule cell; GOC, Golgi cell; MF, mossy fiber; PC, Purkinje cell; SC, stellate cell. Scale bars: 40 μm.