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Fig. 1
Zebrafish EYS protein was concentrated near the connecting cilium/transition zone. Zebrafish eye sections were immunostained with EYS antibody and PNA, and 1D4 antibody and antibodies against acetylated α-tubulin and γ-tubulin. The sections were counter stained with DAPI to show nuclei. (A) EYS antibody staining (green fluorescence) of wild-type retina (n=10). EYS immunoreactivity exhibited as a punctate pattern between the RPE and ONL. (B) EYS immunostaining of EYS-deficient retina (n=10). Note the absence of punctate fluorescence. (C,D) EYS immunostaining of light- and dark-adapted retinas. Note that some of the EYS punctate were located at the basal end of autofluorescence of rod outer segment (asterisks). The domain of EYS punctate shrank upon dark adaptation. (E,F) Double staining of EYS antibody with PNA and 1D4 antibody, respectively. Note that some of the EYS punctate were located on the basal end of the PNA-positive cone outer segment and 1D4-positive outer segment of long double cones (arrows). (G-L) Double staining of EYS (green) and acetylated α-tubulin (red). Note that EYS immunoreactivity is located on the basal end of acetylated α-tubulin. Arrows in G indicate examples at low magnification. (M-N) Double staining of EYS (green) and γ-tubulin (red) antibodies. Note that EYS is apposed to but apical of γ-tubulin. Scale bar in F: 20 µm for A-F. DCOS, double cone outer segment; GCL, ganglion cell layer; INL, inner nuclear layer; LSCOS, long single cone outer segment; ONL, outer nuclear layer; ROS, rod outer segment; RPE, retinal pigment epithelium; SSCOS, short single cone outer segment.