ZFIN Image: Matrone et al., 2016, Fig. 2

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Figure Caption

Fig. 2

Analysis of zebrafish embryo phenotype following exposure to flavopiridol or morpholino injection. (A) Zebrafish embryos at 72 hpf continuously exposed to flavopiridol in the range 1–5µM from 24 hpf up to 120 hpf (at least n = 100 per group), or injected with morpholino 0.2ng/embryo (at least n = 100 per group). (B) Stacked column chart showing phenotypic traits observed following CDK9 inhibition. (C) Flavopiridol dose-dependent inhibition of CDK9 activity. Western blotting for Phospho Serine2 in the Carboxy-Terminal Domain (P-Ser2-CTD) of the RNA pol II. Serine 2 in this complex is phosphorylated by CDK9 when this is active. Tubulin was used as loading control.

Figure Data

Phenotype details

Acknowledgments

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