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Fig. S4
Proliferation in the aorta.
kdrl:mCherry; cmyb:GFP fish were injected with 10mM EdU at 26 hpf and examined by confocal microscopy for GFP+;mCherry+ cells that had proliferated (EdU+, purple) by 32 hpf (A. n=10) or 30 hpf (B. n=10). Open arrows indicate nascent HSPCs, while closed arrows indicate HSPCs that are undergoing EHT. FACS histograms in control (C) and Wnt9a morphant (D) fish sorted for G1 (mCherry+) and endothelium (GFP+). Note the increase in endothelial cells in the G1 fraction of wnt9a morphants. Scale bars= 30µm. Error bars represent standard deviation.
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