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Fig. S1
Documentation of morpholino effect. (A-B) Quantitative PCR expression of product with 3' primer in deleted exon, showing markedly decreased expression of add3a (A) and xpnpep1 (B). (C) Gallbladder PED-6 uptake from control, add3a MO-injected, and add3a MO and mRNA-injected larvae, showing statistically significant rescue of the add3a MO phenotype by add3a mRNA injection. (D) Confocal projection of liver cytokeratin staining of mRNA-rescued 5 dpf larva, showing an essentially normal phenotype. *p≤0.0001, chi square test, MO- + mRNA-injected vs. MO-injected.
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Full text @ J. Pediatr. Gastroenterol. Nutr.