ZFIN Image: van der Vaart et al., 2014, Fig. 3

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Figure Caption

Fig. 3

dram1 Expression during Mycobacterial Infection Is Independent of p53 but Dependent on NF-κB

(A-C) Expression levels of dram1 were determined by qPCR for (A) wild-type, p53^-/-, and myd88^-/- embryos 4 days after infection with Mm, relative to mock-injected controls; (B) wild-type and p53^-/- embryos at 5 dpf after 24 hr of treatment with roscovitine, relative to untreated controls (see also Figure S3); and (C) 1 dpf embryos at 2 hpi with LPS, relative to their respective PBS-injected controls (left panel: wild-type embryos with or without NAI treatment [4 hr total, including 2 hr pretreatment]; middle panel: myd88^-/- embryos; right panel: p53^-/- embryos).

(D) Expression levels of saa were determined by qPCR under the same conditions as those described for (C).

All graphs show data (mean ± SEM) from three biological replicates with n = 20 embryos pooled per replicate. See also Figures S2 and S3.

Acknowledgments

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