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Fig. S3
Bmp signaling is dependent on Acsl4a, related to Figure 2.
Representative western blots of C-Terminal phosphorylated (pSmadC-Term) and pan Smad1/5/8 (SmadTotal) from control embryos and embryos injected with alk8CA mRNA (20-40 pg), acsl4a MO (500 fmol). Anti-BAP/MAK32 is shown as a loading control. (A) Lysates from sphere-stage embryos. (B) Lysates from shield-stage embryos.
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Acknowledgments
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Reprinted from Developmental Cell, 27(6), Miyares, R.L., Stein, C., Renisch, B., Anderson, J.L., Hammerschmidt, M., and Farber, S.A., Long-Chain Acyl-CoA Synthetase 4A Regulates Smad Activity and Dorsoventral Patterning in the Zebrafish Embryo, 635-647, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell