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Fig. S4
Quantitative RT-PCR analysis of dusp6 activation levels in Tg(hsp70-HRASG12V) at various time points after 1 hour of heat shock. Transgenic embryos were kept at 28.5°C until 22 hpf. Some received 37°C heat shock for 1 hour; some were kept as controls. At 4, 6, 8 and 24 hours post-heat shock, the embryos were harvested for RNA extraction and RT-PCR analysis. Transgenic embryos kept at 28.5°C without heat shock were used as controls.
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