ZFIN Image: Cox et al., 2016, Fig. 3

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Figure Caption

Fig. 3

SepH deficient larvae are prone to oxidative stress. (A) Fluorescent detection of H₂O₂ (PF2 fluorescence) in WT and seph mutant larvae at 5 dpf. (Scale bar: 200 µm.) (B) Quantification of H₂O₂ in WT and seph mutant larvae at 5 dpf. n = 5. **P < 0.01. (C) Morphological assessment of WT and seph mutant larvae exposed to menadione (1 µM) from 3 to 5 dpf. (Scale bar: 200 µm.) (D) Quantification of phenotype (unaffected, moderate, or severe) observed following menadione (1 µM) exposure from 3 to 5 dpf. Moderately affected larvae include minor defects, such as edema and tail curling, whereas severely affected larvae include major defects, such as widespread tissue necrosis (loss of transparency).

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA