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Fig. 7
Liver differentiation and outgrowth depends on Nr5a2 activity. (A) Cpd3 treatment reduces the size and alters the position of the fabp10a liver. (B) Quantification of liver size by area of fabp10a expression at 72 hpf. Cpd3 treatment from 12 to 32 hpf enhances the size of the mature liver relative to vehicle (DMSO) treated controls (n>27; p=0.0015; unpaired t-test). Treatment from 28 to 72 hpf and 50-72 hpf reduces the median liver size relative to vehicle (DMSO) treated controls (n>27; p=0.0124; p=0.0007; unpaired t-test). (C) Quantification of liver bud size by area of fluorescence expression in the Tg(prox1a:Citrine) reporter line at 72 hpf. Cpd3 treatment from 50 to 72 hpf significantly reduces the size of the prox1+ liver (n>13; p=0.0136). (D) Fluorescent microscopy images of the embryonic liver in Tg(prox1a:Citrine) reporters following vehicle (DMSO) or Cpd3 treatment from 50 to 72 hpf. (E) Confocal volumetric analysis of liver volume in control (DMSO) and Cpd3 treated (50-72 hpf) Tg(fabp10a:GFP) lines at 72 hpf demonstrates that Cpd3 treatment significantly reduces the volume of the liver (n>3; p=0.0166). (F) Representative 3D renderings of 72 hpf GFP+ livers from the Tg(fabp10a:GFP) line that were exposed to control DMSO or Cpd3 treatment from 50 to 72 hpf. Cpd3 (50-72 hpf) livers are visibly smaller than the control livers.
Reprinted from Developmental Biology, 418(1), Nissim, S., Weeksb, O., Talbot, J.C., Hedgepeth, J.W., Wucherpfennig, J., Schatzman-Bone, S., Swinburne, I., Cortes, M., Alexa, K., Megason, S., North, T.E., Amacher, S.L., Goessling, W., Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development, 108-23, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.