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Fig. 2
Genetic modulation of Nr5a2 disrupts the formation of the mature exocrine pancreas and liver. (A) The oz3 genetic loss of function mutation is a two base pair insertion within exon 3 of the nr5a2 transcript, before the DNA-binding domain and ligand-binding domain. (B) trypsin in situ hybridization at 84 and 120 hpf reveals that nr5a2oz3/oz3 embryos do not develop a mature exocrine pancreas. (C) Quantification of exocrine pancreas size in nr5a2+/+, nr5a2+/oz3, and nr5a2oz3/oz3 embryos by trypsin area calculations. nr5a2oz3/oz3 and nr5a2+/oz3 embryos have a significantly reduced exocrine pancreas size relative to nr5a2+/+ siblings at 84 hpf (n>8 per group; for nr5a2+/+vs. nr5a2+/oz3 (p=0.0282; for nr5a2+/+vs. nr5a2oz3/oz3, p<0.0001; unpaired t-test). (D) Quantification of exocrine pancreas size in nr5a2+/+, nr5a2+/oz3, and nr5a2oz3/oz3 embryos by trypsin area calculations. At 120 hpf, nr5a2oz3/oz3 have a significantly reduced exocrine pancreas size relative to nr5a2+/+ siblings (n>2 per group; p=0.0068; unpaired t-test). (E) In situ hybridization for insulin demonstrates that nr5a2+/+, nr5a2+/oz3, and nr5a2oz3/oz3 embryos develop an endocrine pancreas. (F) In situ hybridization for fabp10a reveals that nr5a2oz3/oz3 embryos have a reduced liver size at 72 and 96 hpf. (G) Quantification of liver size by fabp10a area calculations demonstrates that nr5a2oz3/oz3 embryos have a significantly reduced liver size relative to nr5a2+/+ and nr5a2+/oz3 categories (n>3 per group; p=0.001; unpaired t-test).
Reprinted from Developmental Biology, 418(1), Nissim, S., Weeksb, O., Talbot, J.C., Hedgepeth, J.W., Wucherpfennig, J., Schatzman-Bone, S., Swinburne, I., Cortes, M., Alexa, K., Megason, S., North, T.E., Amacher, S.L., Goessling, W., Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development, 108-23, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.