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Fig. 3
mespaa over-expressing cells do not induce expression of a gata5-EGFP reporter until after gastrulation. (A-D) Host embryos with transplanted WT or mespaa over-expressing donor cells (gata5:EGFP, labeled with rhodamine dextran) at the bud stage (A-B) and the 7 somite stage (12 hpf) (C-D). (E-J) FACS plot of rhodamine dextran-labeled gata5:EGFP donor cells from host embryos at different stages. Cells were either WT (E,G,I) or mespaa over-expressing (F,H,J). Percentage of cells in each bin (GFP +′ve vs ′ve) is shown.
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Reprinted from Developmental Biology, 418(1), Deshwar, A.R., Onderisin, J.C., Aleksandrova, A., Yuan, X., Burrows, J.T., Scott, I.C., Mespaa can potently induce cardiac fates in zebrafish, 17-27, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.