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Fig. 7
Effects of vitamin D receptor (VDR)a and VDRb morpholino oligonucleotides (MOs) on cyp27b1 and cyp24a1 mRNA expressions in 3-d post-fertilization (dpf) zebrafish embryos treated with 1α,25(OH)2D3 (20 µg/l).
(A) cyp27b1 mRNA expression. (B) cyp24a1 mRNA expression. mRNA expressions were analyzed by a qPCR using β-actin as the internal control. Different letters indicate a significant difference (p<0.05) using one-way ANOVA followed by Tukey’s multiple-comparison test. Values are the mean ± SD. (n = 5-6).
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