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Fig. 2
Expression of zebrafish tac3a, tac3b, tac3ra, or tac3rb mRNA in various parts of the brain (A) and changes in zebrafish tac3a at various ages toward puberty (B) as determined by real-time PCR. The relative abundance of the mRNAs was normalized to the amount of elongation factor 1 α (ef1α) by the comparative threshold cycle method; the comparative threshold reflects the relative amount of the transcript. Results are means ± SEM (n = 11-15). Means marked with different letters differ significantly (P < 0.05).
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Full text @ Proc. Natl. Acad. Sci. USA