Fig. 1
Leg1a and Leg1b are two closely related homologs in zebrafish.
(A) Diagram showing the arrangement of leg1a and leg1b on chromosome 20. (B) RT-PCR showing the expression of leg1a and leg1b in adult liver. Primer pairs amplifying leg1a are derived from exon1 of leg1a (lanes 1 and 2), leg1b are from exon1 and exon2 of leg1b (lanes 3 and 4). Lanes 1 and 3: cDNA as template; lanes 2 and 4: genomics DNA as template. (C) Diagram showing leg1a and leg1b genomic structures. Red box: exon; uneven line: introns. (D) Alignment of Leg1a and Leg1b amino acid sequences to highlight the 39 different amino acids between them (boxed in red).