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Fig. S6
Interaction of g9a and znf644 genes in regulating progenitor cell cycle. (A) WISH assays monitoring the expression of ccnd1 in (top) midbrain or (bottom) retinal cross-sections at 48 hpf from WT embryos or embryos injected with the indicated combined subthreshold MO doses. (B) Immunostaining assays monitoring pH3 levels or BrdU incorporation at 48 hpf from WT or embryos injected with the indicated combined subthreshold MO doses. (C) Rescue experiments in which mRNA encoding WT human (ZNF644) or mutant version lacking G9a/GLP binding capability (C1263A) is coinjected with znf644a-MO (left) or znf644b-MO (right). Vsx2 expression was assessed by WISH at 48 hpf, and PCNA, pH3, and Zpr1 levels (n=3 in each group) were assessed by immunostaining and confocal microscopy at the indicated time points.